MyBioSource.com's SARA anchor protein is a Rabbit Polyclonal antibody. This antibody has been shown to work in applications such as: Immunoprecipitation, and Western Blot. The SARA anchor protein Antibody was generated using ZFYVE9 as the antigen and it reacts with Drosophila/Arthropod, and Xenopus/Amphibian.
Description
The transforming growth factor-beta (TGF-beta) super-family of proteins signaling involves the activation of TGF beta receptor activation by ligand binding that initiates the constitutively active type II receptor kinase to come in close proximity to its substrate, the type I receptor kinase (1). The kinase activity from the type II receptor phosphorylates the type I receptor. The type I receptor in turn phosphorylates one of the several transcription factor proteins (Smad). The activated Smads are then translocated to the nucleus and regulates the gene expression. The Smads are recruited to the receptor complex by an anchor protein, SARA (Smad anchor for Receptor Activation). In the nucleus, Smads regulate transcriptional responses by recruiting co-activators and co-repressors to a wide array of DNA-binding partners. The SARA is also known as Smad2 binding proteins. Thus, Smads function as transcriptional co-modulators to regulate TGFbeta-dependent gene expression. Therefore, the role of SARA in Smad signaling pathway is critical for transmitting transforming growth factor-beta (TGF-beta) super family signals from the cell surface to the nucleus (2). The SARA protein is also associated with several protein phosphatases that dephosphorylate and truncates the TGF beta signal transduction. One of the protein phosphatase that has been recently identified is PP1c. The SARA has a PP1c binding motif, disruption of this motif hyper phosphorylation of type 1 receptor and stimulated expression of a target of TGF beta signaling (1). Smad anchor for Receptor Activation (SARA) in drosophila melanogaster is a 1343 amino acid long protein with an apparent molecular weight of 165-167 kDa. The human homologue of this protein "Mothers Against decapentaplegic homologue interacting protein (MADI_human) is larger than SARA (1425 amino acids) with at least two variants reported (MAD1 and MAD 2). The Anti-SARA-selective antibodies were generated against conserved sequences near the N-terminal, mid-region and the C-terminal end of the proteins that are unique to SARA protein. The SARA selective antibodies were affinity purified against immobilized antigen based affinity chromatography and is epitope-specific antibodies. The polyclonal antibody strongly labels a 165-170kDa protein in D. melanogaster extracts. Anti-SARA-selective antibodies are also available in affinity-purified form for confocal, Western blotting and immunocytochemical analyses. MyBioSource will also conjugate antibodies with fluorescent probes upon request at extra charge. MyBioSource also provides antibodies against proteins that are involved Drosophila melanogaster research such as several Anti-PDE antibodies, Anti-Wishfulthinking, Anti-Baboon, Anti-Shadow, Anti-Shade, Anti-Saxophone, Anti-Phantom and Anti-Punt protein. MyBioSource employs cyclic peptide methodology for generating antibodies, which results in higher titer and specificity (6). MyBioSource, will also provide Western blot positive controls for most of these antibodies in ready-to-use buffer for easy identification of respective proteins. Limited quantities of antigens are also available. Please enquire for their availability before ordering